Center for Molecular Analysis of Disease Pathways (CMADP)

An NIH Center of Biomedical Research Excellence (COBRE)

The development of new enabling technologies is critical to improving our understanding and treatment of disease. The Center for Molecular Analysis of Disease Pathways (CMADP), an NIH Center for Biomedical Research Excellence (COBRE), brings together junior and senior faculty from the physical, biological, and pharmaceutical sciences at the University of Kansas and other academic institutions in Kansas to conduct multidisciplinary research to develop and implement cutting-edge technologies for elucidating the genetic, chemical, and physical mechanisms of biological processes involved in disease.

MRB Photo

Microfabrication & Microfluidics Core

The scientific emphasis of the Center is on the creation and implementation of enabling technologies that can be employed to identify new therapeutic targets. This includes state of the art methods for gene sequencing, the genetic manipulation of model organisms, custom fluorescent molecular probes for monitoring physiological processes in model organisms, and microfluidic systems for manipulation and monitoring of biochemical pathways.

CMADP capitalizes on existing and evolving expertise in the fields of bioanalytical chemistry, molecular design, genomics and bioengineering at the University of Kansas (KU) and Kansas State University (KSU). The PI, Co-Investigators, research investigators, core directors, mentors, and members of the internal advisory committee represent ten academic departments, six Colleges and Schools, and two Research Centers at KU and KSU. The departments of Biology and Chemistry at KSU are also represented by members of the CMADP's core facility steering committees. The senior faculty members in these departments, schools and centers have a long history of research collaborations and cross-training of graduate students and post-doctoral associates.

Research reported in this Web site was supported by an Institutional Development Award (IDeA) from the National Institute of General Medical Sciences of the National Institutes of Health under Award Number P20GM103638. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.


Recent News

February 2017
CMADP Project Investigators co-author Top Downloaded article in Lab on a Chip

CMADP Co-I awarded R01 from NIH National Cancer Institute

CMADP Graduate's research featured on cover of Genetics and in other journals

October 2016
CMADP Co-I receives Mathers Foundation grant

View all news »

Upcoming Events
Special seminar by Dr. James P. Landers
Commonwealth Professor in Chemistry,
Mechanical Engineering & Pathology
University of Virginia

Wednesday, May 17, 2017 at 3:00pm
Simons Auditorium, HBC, West Campus

"Integrated Microfluidic Systems for Forensic DNA Analysis"
In 2006, we demonstrated that microfluidic technology could provide a ‘lab-on-a-chip’ solution for real-world genetic analysis. Sample-in/answer-out functionality was shown for the detection of bacteria in mouse blood and in a human nasal swab, with a sub-30 minute analytical time for DNA extraction, amplification, electrophoretic separation and detection. We extrapolated these technology developments to the analysis of short tandem repeats (STR) in human DNA; these clinically-insignificant (presumably) tetranucleotide sequences function effectively for statistically-relevant matching in human identification. Our efforts led to the development of a commercializable system designed for implementation in crime labs for STR profiling convicted felons or, in some states, profiling arrestees in booking stations. An intricate but functional microfluidic architecture allowed sample-to-profile to be achieved from a cheek swab in less than 80 minutes, using nanoliter flow control, infrared thermocycling and rapid electrophoretic separation of DNA with 5-color fluorescence detection. We have since demonstrated the fabrication of hybrid microdevices composed of inexpensive polymeric materials, many of these commercial-off-the-shelf. We have designed, built and functionalized fully-integrated DNA analysis chemistry/microfluidics on a rotationally-driven system the size of a compact disc. With this system, DNA can be extracted from a swab, PCR amplified to generate an abundance of DNA fragments of the STR loci, followed by resolution of those fragments in a separation in a 4 cm Leff channel that is complete in <300 sec with a 2-base resolution. The processes that allow for swab in–profile out microfluidics are carried out on an instrument that can be carried in one hand and weighs ~14 lbs, ultimately allowing for facile rapid human identification/screening in the field.
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