Probing Lipid-Protein Interactions in Biological Self-Assembly

Prof. Prajnaparamita Dhar, Department of Chemical & Petroleum Engineering
University of Kansas

Project dates: 2012-2015

Mentor: Stevin Gehrke

The optimal cholesterol content in replacement surfactants is currently under debate, primarily because of a lack of understanding of the role of cholesterol in the mechanical and morphological properties of LS monolayers. These mechanical properties, in turn, regulate the surface tension lowering and adsorption properties of the surfactant. The primary goal of Dr. Dhar’s research is to determine, for the first time, the physiologically optimal surface viscosity of the lung surfactant using an active microrheology technique unique to our lab.

She hypothesizes that there exists an optimal surface viscosity in an effective lung surfactant that provides both rapid adsorption to the air-water interface and ultra-low surface tensions. Professor Dhar’s goal is to determine how best to achieve this optimum by controlling the cholesterol fraction of a synthetic replacement lung surfactant. Three orders of magnitude increased sensitivity can be achieved with her microrheology technique as compared to macroscopic rheometers. This allows precise monitoring of changes in the molecular organization of the lung surfactant film in the presence of cholesterol and enables accurate measurements of surface viscosity of surfactant films.

Ultimately, determining the optimal cholesterol concentration will enable a better design of synthetic surfactants to treat Neonatal Respiratory Distress Syndrome (NRDS) and may give insights into the causes of surfactant inactivation in Adult Respiratory Distress Syndrome (ARDS).

Recent News

June 2018
Mei He's review article selected for journal cover of Lab on a Chip

May 2018
Arghya Paul earns departmental teaching award
Yong Zeng promoted to associate professor with tenure

March 2018
Sue Lunte to receive 2018 ANACHEM Award
Christian Ray selected to co-organize international conference

View all news »

Upcoming Events


KU Today