Microfluidic engineering of immunogenic exosomes for personalized cancer vaccine

Prof. Mei He, Department of Biological & Agricultural Engineering
Kansas State University

Project dates: 2016-2017

Exosome, as a natural and safe therapeutic delivery system, is emerging in cancer immunotherapy, yet hard to harvest as a pure, immunogenic population. Consequently, co-purified exosome subtypes and extracellular microvesicles could confound our understanding on activation of immune pathways and effective cancer vaccination. This project specifically addresses technology challenges by introducing a lowcost microfluidic approach for large-scale production of clinical-grade immunogenic exosomes, and examining the roles of immunogenic exosomes in MHC-I antigen presentation pathway and antitumor responses. The study focuses on the immunogenicity of peptide-engineered exosomes in antitumor responses. The long term goal is to establish an enabling strategy for studying immunogenic roles of variable peptide-engineered exosomes in cancer immunotherapy and developing personalized cancer vaccines. Microfluidic high-throughput isolation of MHC-I specific exosomes will be uniquely streamlined with vesicular loading of antigenic peptides, and subsequent light-triggered release of intact, engineered exosomes in continuous-flow. The engineered immunogenic exosomes will be assessed to the degree of potency and activity in stimulating immune pathways that are critical for an effective antitumor response in vitro (e.g., CD8+ cytotoxic T cells and CD4+ T helper cells stimulating). Outcomes will increase understanding of fundamentals and roles of variable peptide-pulsed immunogenic exosomes in immune system, provide well defined models for in vivo study of immunogenic exosomes in tumor microenvironment, and gain knowledge of cell-free cancer vaccination system for designing personalized cancer vaccines.


Recent News

May 2017
CMADP Research Project Investigator co-recipient of NCI R33 grant

April 2017
CMADP Graduate, one of ACS's 'Must See Presenters', speaks on latest "chemobrain" findings at Spring 2017 ACS National Meeting

February 2017
CMADP Project Investigators co-author Top Downloaded article in Lab on a Chip

CMADP Graduate's research featured on cover of Genetics and in other journals

View all news »

Upcoming Events

TBA

KU Today